Diversidad de nematodos marinos de Chile continental y antártico: una evaluación morfológica y molecular
Date
2014Author
Valderrama-Aravena, Natalia
Pérez-Araneda, Karla
Avaria Llautureo, Jorge
Hernández, Cristián E.
Lee, Matthew
Brante, Antonio
Publisher
ScieloDescription
Artículo de publicación ISIMetadata
Show full item recordAbstract
Free-living nematodes are an important component of the marine benthos. In Chile there have been few studies
on this group, and the majority has focused on the morphological aspect only. In this study, ribosomal 18S RNA and
mitochondrial COI genes were used as genetic markers to complement morphological analyses to study the continental
and Antarctic Chilean marine nematofauna. Different protocols for fixing, extracting and amplifying DNA were also tested.
Not all the possible combinations produced good results. In fact, only the 18S gene showed consistently results. Phylogenetic
analyses showed some discordance between classical taxonomy (i.e., based on morphology) and molecular data. These
results suggest that taxonomic classification using integrative approaches including morphological characteristics and
molecular information is needed to study the diversity and evolution of this complex group.